| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Shao, Emma X (7)
|
ADGUY |
25.91 |
24.36 |
-1.55 |
| 2 |
MacLeod, Angelina M (8)
|
ADUSAM |
25.57 |
24.82 |
-0.75 |
| 3 |
Perri, Gabriella M (8)
|
ADDELM |
|
26.03 |
- |
| 4 |
Johnston, Layla A (8)
|
ADGLFY |
29.76 |
26.83 |
-2.93 |
| 5 |
Braun, Julia L (7)
|
ADNTS |
36.87 |
27.11 |
-9.76 |
| 6 |
Downey, Isabelle G (8)
|
ADSARY |
32.15 |
27.58 |
-4.57 |
| 7 |
Huang, Rylie Z (8)
|
ADSSCY |
34.82 |
29.49 |
-5.33 |
| 8 |
Zhang, Annabelle Y (7)
|
ADCPP |
|
29.65 |
- |
| 9 |
Zen, Dominique A (7)
|
ADDELM |
|
29.81 |
- |
| 10 |
Stoup, Madelyn L (8)
|
ADCPP |
30.94 |
30.03 |
-0.91 |
| 11 |
Relyea, Cadence G (7)
|
ADDELM |
|
30.20 |
- |
| 12 |
Woytowich, Emma J (8)
|
ADSCHE |
|
34.35 |
- |
| 13 |
Dainotto, Olivia F (8)
|
ADCLIN |
|
42.99 |
- |
|
O'Donnell, Bridgett M (7)
|
ADEELS |
37.13 |
DQ |
- |
|
Sekelj, Ella S (6)
|
ADNTS |
|
DQ |
- |
|
Hovak, Madelyn E (8)
|
ADNHA |
|
DQ |
- |
|
Laurie, Josephine S (8)
|
ADHAWK |
34.51 |
DQ |
- |
|
Adams, Michelle E (6)
|
ADCPP |
|
DQ |
- |
|
Pickering, Michelle E (8)
|
ADBSPA |
34.62 |
DQ |
- |
|
Brandl, Kristin W (8)
|
ADBSPA |
|
DQ |
- |
|
D'Amato, Victoria (8)
|
ADALBS |
33.94 |
NS |
- |